Click the PMID link to go the the PubMed listing for each paper. Papers containing work funded by the US National Institutes of Health are available for free in the PubMed Central repository. For other papers or book chapters, please e-mail to request a pdf.


    • Fukushima CT, Dancil IS, Clary H, Shah N, Nadtochiy SM, Brookes PS. (2024) Reactive Oxygen Species Generation by Reverse Electron Transfer at Mitochondrial Complex I Under Simulated Early Reperfusion Conditions. Redox Biol. 70: 103047. PMID 38295577
    • Beutner G, Burris JR, Collins MP, Kulkarni CA, Nadtochiy SM, de Mesy Bentley KL, Cohen ED, Brookes PS, Porter GA Jr. (2024) Coordinated metabolic responses to cyclophilin D deletion in the developing heart. iScience 27(3): 109157. PMID 38414851


    • Sautchuk R Jr, Yu C, McArthur M, Massie C, Brookes PS, Porter GA Jr, Awad H, Eliseev RA. Role of the Mitochondrial Permeability Transition in Bone Metabolism and Aging J. Bone Miner. Res. (2023) 38: 522-540. PMID 36779737
    • Milliken AS, Ciesla JH, Nadtochiy SM, Brookes PS. Distinct effects of intracellular vs. extracellular acidic pH on the cardiac metabolome during ischemia and reperfusion. J. Mol. Cell. Cardiol. (2023) 174: 101-114. PMID 36481511
    • Paine A, Brookes PS, Bhattacharya S, Li D, De La Luz Garcia-Hernandez M, Tausk F, Ritchlin C. Dysregulation of Bile Acids, Lipids, and Nucleotides in Psoriatic Arthritis Revealed by Unbiased Profiling of Serum Metabolites. Arthritis Rheumatol. (2023) 75: 53-63. PMID 35818333


    • Milliken AS, Nadtochiy SM, Brookes PS. Inhibiting Succinate Release Worsens Cardiac Reperfusion Injury by Enhancing Mitochondrial Reactive Oxygen Species Generation J. Am. Heart. Assoc. (2022) 11(13):e026135. PMID 35766275.


    • Kotla S, Zhang A, Imanishi M, Ko KA, Lin SH, Gi YJ, Moczygemba M, Isgandarova S, Schadler KL, Chung C, Milgrom SA, Banchs J, Yusuf SW, Amaya DN, Guo H, Thomas TN, Shen YH, Deswal A, Herrmann J, Kleinerman ES, Entman ML, Cooke JP, Schifitto G, Maggirwar SB, McBeath E, Gupte AA, Krishnan S, Patel ZS, Yoon Y, Burks JK, Fujiwara K, Brookes PS, Le NT, Hamilton DJ, Abe JI. (2021) Nucleus-mitochondria positive feedback loop formed by ERK5 S496 phosphorylation-mediated poly (ADP-ribose) polymerase activation provokes persistent pro-inflammatory senescent phenotype and accelerates coronary atherosclerosis after chemo-radiation. Redox Biol. 47: 102132. PMID 34619528
    • Brookes PS, Jimenez AG. (2021) Metabolomics of aging in primary fibroblasts from small and large breed dogs. Geroscience 43: 1683-1696. PMID 34132979
    • Cohen ED, Yee M, Porter GA Jr, Ritzer E, McDavid AN, Brookes PS, Pryhuber GS, and O’Reilly MA. (2021) Neonatal hyperoxia inhibits proliferation and survival of atrial cardiomyocytes by suppressing fatty acid synthesis. JCI Insight. 6: e140785. PMID 33507880
    • Nadtochiy SM, Ngai M, Brookes PS. (2021) Modified Blue Native Gel Approach for Analysis of Respiratory Supercomplexes. Methods Mol Biol. 2276: 227-234. PMID 34060045
    • Lim Y, Berry B, Viteri S, McCall M, Park EC, Rongo C, Brookes PS, Nehrke K. Autophagy. (2021) FNDC-1-mediated mitophagy and ATFS-1 coordinate to protect against hypoxia-reoxygenation. Autophagy 19: 1-13. PMID 33416042


    • Trippier PC, Huwaimel BI, Bhakta M, Kulkarni CA, Milliken AS, Wang F, Peng A, Brookes PS. (2020) Discovery of Halogenated Benzothiadiazine Derivatives with Anticancer Activity. Chem Med Chem. 16: 1143-1162. PMID: 33331124
    • Milliken AS, Kulkarni CA, Brookes PS. (2020) Acid enhancement of ROS generation by complex-I reverse electron transport is balanced by acid inhibition of complex-II: Relevance for tissue reperfusion injury. Redox Biol. 37: 101733. PMID 33007502
    • Kulkarni CA, Nadtochiy SM, Kennedy L, Zhang J, Chhim S, Alwaseem H, Murphy E, Fu D, Brookes PS. (2020) ALKBH7 Mediates Necrosis via Rewiring of Glyoxal Metabolism. Elife. 2020 9: e58573. PMID 32795389
    • Milliken AS, Brookes PS. (2020) Amber Alert: Getting to the Heart of Succinate Efflux in Reperfusion Injury. Cardiovasc Res. cvaa216 PMID 32666087
    • Bennett JA, Mastrangelo MA, Ture SK, Smith CO, Loelius SG, Berg RA, Shi X, Burke RM, Spinelli SL, Cameron SJ, Carey TE, Brookes PS, Gerszten RE, Sabater-Lleal M, de Vries PS, Huffman JE, Smith NL, Morrell CN, Lowenstein CJ. (2020) The choline transporter Slc44a2 controls platelet activation and thrombosis by regulating mitochondrial function. Nat. Commun. 11: 3479. PMID 32661250
    • Zuurbier CJ, Bertrand L, Beauloye CR, Andreadou I, Ruiz-Meana M, Jespersen NR, Kula-Alwar D, Prag HA, Eric Botker H, Dambrova M, Montessuit C, Kaambre T, Liepinsh E, Brookes PS, Krieg T. J. (2020) Cardiac metabolism as a driver and therapeutic target of myocardial infarction. Cell. Mol. Med. 24: 5937-5954. PMID 32384583
    • Chapa-Dubocq XR, Rodríguez-Graciani KM, Guzmán-Hernández RA, Jang S, Brookes PS, Javadov S. (2020) Cardiac Function is not Susceptible to Moderate Disassembly of Mitochondrial Respiratory Supercomplexes. Int J Mol Sci. 21. pii: E1555. PMID 32106430


    • Burke CG, Myers JR, Boule LA, Post CM, Brookes PS, Lawrence BP. (2019) Early life exposures shape the CD4+ T cell transcriptome, influencing proliferation, differentiation, and mitochondrial dynamics later in life. Sci Rep. 9: 11489. PMID 31391494
    • Wang YT, Lim Y, McCall MN, Huang KT, Haynes CM, Nehrke K, Brookes PS. (2019) Cardioprotection by the mitochondrial unfolded protein response requires ATF5. Am J Physiol Heart Circ Physiol. 317: H472-H478. PMID 31274354
    • Lim Y, Rubio-Peña K, Sobraske PJ, Molina PA, Brookes PS, Galy V, Nehrke K. (2019) Fndc-1 contributes to paternal mitochondria elimination in C. elegans. Dev Biol. 454: 15-20. PMID 31233739
    • Kulkarni CA, Brookes PS. (2019) Cellular Compartmentation and the Redox/Nonredox Functions of NAD+. Antioxid Redox Signal. 31: 623-642. PMID 30784294


    • Nadtochiy SM, Wang YT, Nehrke K, Munger J, Brookes PS. (2018) Cardioprotection by nicotinamide mononucleotide (NMN): Involvement of glycolysis and acidic pH. J Mol Cell Cardiol. 121: 155-162. PMID 29958828
    • Smith CO, Wang YT, Nadtochiy SM, Miller JH, Jonas EA, Dirksen RT, Nehrke K, Brookes PS. (2018) Cardiac metabolic effects of KNa1.2 channel deletion and evidence for its mitochondrial localization. FASEB J. 32(11): fj201800139R. PMID 29863912
    • Zhang J, Wang YT, Miller JH, Day MM, Munger JC, Brookes PS. (2018) Accumulation of Succinate in Cardiac Ischemia Primarily Occurs via Canonical Krebs Cycle Activity. Cell Rep. 23: 2617-2628. PMID 29847793
    • Peoples JNR, Maxmillian T, Le Q, Nadtochiy SM, Brookes PS, Porter GA Jr, Davidson VL, Ebert SN. (2018) Metabolomics reveals critical adrenergic regulatory checkpoints in glycolysis and pentose-phosphate pathways in embryonic heart. J Biol Chem. 293: 6925-6941. PMID 29540484
    • Sahni PV, Zhang J, Sosunov S, Galkin A, Niatsetskaya Z, Starkov A, Brookes PS, Ten VS. (2018) Krebs cycle metabolites and preferential succinate oxidation following neonatal hypoxic-ischemic brain injury in mice. Pediatr Res. 83: 491-497. PMID 29211056


    • Brookes PS, Taegtmeyer H. (2017) Metabolism: A Direct Link Between Cardiac Structure and Function. Circulation. 136: 2158-2161.
      PMID 29180494
    • Resseguie EA, Brookes PS, O’Reilly MA. (2017) SMG-1 kinase attenuates mitochondrial ROS production but not cell respiration deficits during hyperoxia. Exp Lung Res. 43: 229-239. PMID 28749708
    • Nadtochiy SM, Wang YT, Zhang J, Nehrke K, Schafer X, Welle K, Ghaemmaghami S, Munger J, Brookes PS. (2017) Potential mechanisms linking SIRT activity and hypoxic 2-hydroxyglutarate generation: no role for direct enzyme (de)acetylation. Biochem J. 474: 2829-2839. PMID 28673962
    • Smith CO, Nehrke K, Brookes PS. (2017) The Slo(w) path to identifying the mitochondrial channels responsible for ischemic protection. Biochem J. 474: 2067-2094. PMID 28600454
    • Wang H, Huwaimel B, Verma K, Miller J, Germain TM, Kinarivala N, Pappas D, Brookes PS, Trippier PC. (2017) Synthesis and Antineoplastic Evaluation of Mitochondrial Complex II (Succinate Dehydrogenase) Inhibitors Derived from Atpenin A5. Chem Med Chem. 12: 1033-1044. PMID 28523727


    • Nadtochiy SM, Schafer X, Fu D, Nehrke K, Munger J, Brookes PS. (2016) Acidic pH Is a Metabolic Switch for 2-Hydroxyglutarate Generation and Signaling. J Biol Chem. 291: 20188-97. PMID 27510037
    • Brand MD, Goncalves RL, Orr AL, Vargas L, Gerencser AA, Borch Jensen M, Wang YT, Melov S, Turk CN, Matzen JT, Dardov VJ, Petrassi HM, Meeusen SL, Perevoshchikova IV, Jasper H, Brookes PS, Ainscow EK. (2016) Suppressors of Superoxide-H2O2 Production at Site IQ of Mitochondrial Complex I Protect against Stem Cell Hyperplasia and Ischemia-Reperfusion Injury.
      Cell Metab. 24: 582-592. PMID 27667666
    • Peña S, Sherman T, Brookes PS, Nehrke K. (2016) The Mitochondrial Unfolded Protein Response Protects against Anoxia in Caenorhabditis elegans. PLoS One. 11: e0159989. PMID 27459203
    • Shum LC, White NS, Nadtochiy SM, Bentley KL, Brookes PS, Jonason JH, Eliseev RA. (2016) Cyclophilin D Knock-Out Mice Show Enhanced Resistance to Osteoporosis and to Metabolic Changes Observed in Aging Bone. PLoS One. 11: e0155709. PMID 27183225
    • Pell VR, Chouchani ET, Murphy MP, Brookes PS, Krieg T. (2016) Moving Forwards by Blocking Back-Flow: The Yin and Yang of MI Therapy. Circ Res. 118: 898-906. PMID 26941425
    • Wojtovich AP, Smith CO, Urciuoli WR, Wang YT, Xia XM, Brookes PS, Nehrke K. (2016) Cardiac Slo2.1 Is Required for Volatile Anesthetic Stimulation of K+ Transport and Anesthetic Preconditioning. Anesthesiology. 24: 1065-76. PMID 26845140
    • Zhang J, Nadtochiy SM, Urciuoli WR, Brookes PS. (2016) The cardioprotective compound cloxyquin uncouples mitochondria and induces autophagy. Am J Physiol Heart Circ Physiol. 310: H29-38. PMID 26519034
    • Klionsky D. et al. (2016) Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy. 12: 1-222. PMID 26799652


    • Nadtochiy SM, Urciuoli W, Zhang J, Schafer X, Munger J, Brookes PS. (2015) Metabolomic profiling of the heart during acute ischemic preconditioning reveals a role for SIRT1 in rapid cardioprotective metabolic adaptation. J Mol Cell Cardiol. 88: 64-72. PMID 26388263
    • Shi X, O’Neill MM, MacDonnell S, Brookes PS, Yan C, Berk BC. (2015) The RSK Inhibitor BIX02565 Limits Cardiac Ischemia/Reperfusion Injury. J Cardiovasc Pharmacol Ther. pii: 1074248415591700. [Epub ahead of print]. PMID 26130615
    • Resseguie EA, Staversky RJ, Brookes PS, O’Reilly MA. (2015) Hyperoxia activates ATM independent from mitochondrial ROS and dysfunction. Redox Biol. 5: 176-85. PMID 25967673
    • Kiebala M, Skalska J, Casulo C, Brookes PS, Peterson DR, Hilchey SP, Dai Y, Grant S, Maggirwar SB, Bernstein SH. (2015) Dual targeting of the thioredoxin and glutathione antioxidant systems in malignant B cells: a novel synergistic therapeutic approach. Exp Hematol. 43: 89-99. PMID 25448488
    • Chouchani ET, Pell VR, Gaude E, Aksentijević D, Sundier SY, Robb EL, Logan A, Nadtochiy SM, Ord EN, Smith AC, Eyassu F, Shirley R, Hu CH, Dare AJ, James AM, Rogatti S, Hartley RC, Eaton S, Costa AS, Brookes PS, Davidson SM, Duchen MR, Saeb-Parsy K, Shattock MJ, Robinson AJ, Work LM, Frezza C, Krieg T, Murphy MP. (2015) Ischaemic accumulation of succinate controls reperfusion injury through mitochondrial ROS. Nature. 515(7527): 431-5. PMID 25383517


    • Galloway CA, Lee H, Brookes PS, Yoon Y. (2014) Decreasing mitochondrial fission alleviates hepatic steatosis in a murine model of nonalcoholic fatty liver disease. Am J Physiol Gastrointest Liver Physiol. 307: G632-41. PMID 25080922
    • Kalifa L, Gewandter JS, Staversky RJ, Sia EA, Brookes PS, O’Reilly MA. (2014) DNA Double-Strand Breaks Activate ATM Independent of Mitochondrial Dysfunction in A549 Cells. Free Radic Biol Med.75:30-9. PMID 25048973
    • Nadtochiy SM, Brookes PS, Porter GA. (2014) SIRT3 deficiency exacerbates ischemia-reperfusion injury: implication for aged hearts. Am J Physiol Heart Circ Physiol. 306: H1602-9. PMID 24748594
    • Nadtochiy SM, Madukwe J, Hagen F, Brookes PS. (2014) Mitochondrial targeted nitro-linoleate, a tool compound for studying cardioprotection. Br. J. Pharmacol. 171: 2091-8. PMID 24102583
    • Brookes PS (2014) Internet publicity of data problems in the bioscience literature correlates with enhanced corrective action. PeerJ 2: e313. PMID 24765564


    • Queliconi BB, Marazzi TB, Vaz SM, Brookes PS, Nehrke K, Augusto O, Kowaltowski AJ. (2013) Bicarbonate Modulates Oxidative and Functional Damage in Ischemia-Reperfusion. Free Radic. Biol. Med. 55: 46-53. PMID 23195687
    • Redman EK, Brookes PS, Karcz MK (2013). Role of p90RSK in regulating the Crabtree effect: implications for cancer. Biochem. Soc. Trans. 41: 124-6. PMID 23356270
    • Lagadinou ED, Sach A, Callahan K, Rossi RM, Neering SJ, Minhajuddin M, Ashton JM, Pei S, Grose V, O’Dwyer KM, Liesveld JL, Brookes PS, Becker MW, Jordan CT. (2013) BCL-2 inhibition targets oxidative phosphorylation and selectively eradicates quiescent human leukemia stem cells. Cell Stem Cell. 12: 329-41. PMID 23333149
    • Wojtovich AP, Urciuoli WR, Chatterjee S, Fisher AB, Nehrke K, Brookes PS. (2013) Kir6.2 is not the mitochondrial KATP channel but is required for cardioprotection by ischemic preconditioning. Am. J. Physiol. 304: H1439-45. PMID 23585131
    • Wojtovich AP, Nadtochiy SM, Urciuoli WR, Smith CO, Grunnet M, Nehrke K, Brookes PS. (2013) A non-cardiomyocyte autonomous mechanism of cardioprotection involving the SLO1 BK channel. Peer J. 1: e48. PMID 23638385
    • Chouchani ET, Methner C, Nadtochiy SM, Logan A, Pell VR, Ding S, James AM, Cochemé HM, Reinhold J, Lilley KS, Partridge L, Fearnley IM, Robinson AJ, Hartley RC, Smith RA, Krieg T, Brookes PS, Murphy MP. (2013) Cardioprotection by S-nitrosation of a cysteine switch on mitochondrial complex I. Nat Med. 19: 753-9. PMID 23708290
    • Gohil VM, Zhu L, Baker CD, Cracan V, Yaseen A, Jain M, Clish CB, Brookes PS, Bakovic M, Mootha VK. (2013) Meclizine inhibits mitochondrial respiration through direct targeting of cytosolic phosphoethanolamine metabolism. J Biol Chem. 288: 35387-95. PMID 24142790
    • Wojtovich AP, Smith CO, Haynes CM, Nehrke KW, Brookes PS. (2013) Physiological consequences of complex II inhibition for aging, disease, and the mKATP channel. Biochim. Biophys. Acta. 1827: 598-611. PMID 23291191


    • Walters AM, Porter GA Jr., Brookes PS. (2012) Mitochondria as a drug target in ischemic heart disease and cardiomyopathy. Circ. Res. 111: 1222-36. PMID 23065345
    • Klionsky DJ, Brookes PS, et al. (2012) Guidelines for the use and interpretation of assays for monitoring autophagy. Autophagy. 8: 445-544. PMID 22966490
    • Guo S, Olm-Shipman A, Walters A, Urciuoli WR, Devito S, Nadtochiy SM, Wojtovich AP, Brookes PS. (2012) A cell-based phenotypic assay to identify cardioprotective agents. Circ Res. 110: 948-957. PMID 22394516
    • Bernstein SH, Venkatesh S, Li M, Lee J, Lu B, Hilchey SP, Morse KM, Metcalfe HM, Skalska J, Andreeff M, Brookes PS, Suzuki CK. (2012) The mitochondrial ATP-dependent Lon protease: a novel target in lymphoma death mediated by the synthetic triterpenoid CDDO and its derivatives. Blood. 119: 3321-3329. PMID 22323447
    • Wojtovich AP, DiStefano P, Sherman T, Brookes PS, Nehrke K. (2012) Mitochondrial ATP-sensitive potassium channel activity and hypoxic preconditioning are independent of an inwardly rectifying potassium channel subunit in Caenorhabditis elegans. FEBS Lett. 586: 428-434. PMID 22281198
    • Nadtochiy SM, Zhu Q, Urciuoli W, Rafikov R, Black SM, Brookes PS. (2012) Nitroalkenes confer acute cardioprotection via adenine nucleotide translocase 1. J Biol Chem. 287: 3573-3580. PMID 22158628


    • Nadtochiy SM, Redman E, Rahman I, Brookes PS. (2011) Lysine deacetylation in ischaemic preconditioning: the role of SIRT1. Cardiovasc Res. 89: 643-649. PMID 20823277
    • Ding B, Gibbs PE, Brookes PS, Maines MD. (2011) The coordinated increased expression of biliverdin reductase and heme oxygenase-2 promotes cardiomyocyte survival: a reductase-based peptide counters ?-adrenergic receptor ligand-mediated cardiac dysfunction. FASEB J. 25: 301-313. PMID 20876213
    • Queliconi BB, Wojtovich AP, Nadtochiy SM, Kowaltowski AJ, Brookes PS. (2011) Redox regulation of the mitochondrial K(ATP) channel in cardioprotection. Biochim Biophys Acta. 1813: 1309-1315. PMID 21094666
    • Shi X, Yan C, Nadtochiy SM, Abe J, Brookes PS, Berk BC. (2011) p90 ribosomal S6 kinase regulates activity of the renin-angiotensin system: a pathogenic mechanism for ischemia-reperfusion injury. J Mol Cell Cardiol. 51: 272-275. PMID 21609719
    • Nadtochiy SM, Yao H, McBurney MW, Gu W, Guarente L, Rahman I, Brookes PS. (2011) SIRT1-mediated acute cardioprotection. Am J Physiol. 301: H1506-H1512. PMID 21856913
    • Wojtovich AP, Nadtochiy SM, Brookes PS, Nehrke K. (2011) Ischemic preconditioning: the role of mitochondria and aging. Exp Gerontol. 47: 1-7. PMID 22100642
    • Wojtovich AP, Sherman TA, Nadtochiy SM, Urciuoli WR, Brookes PS, Nehrke K. (2011) SLO-2 is cytoprotective and contributes to mitochondrial potassium transport. PLoS One. 6: e28287. PMID 22145034


    • Roser KS, Brookes PS, Wojtovich AP, Olson LP, Shojaie J, Parton RL, Anders MW. (2010) Mitochondrial biotransformation of omega-(phenoxy)alkanoic acids, 3-(phenoxy)acrylic acids, and omega-(1-methyl-1H-imidazol-2-ylthio)alkanoic acids: a prodrug strategy for targeting cytoprotective antioxidants to mitochondria. Bioorg Med Chem. 18: 1441-1448. PMID 20129794
    • Gohil VM, Sheth SA, Nilsson R, Wojtovich AP, Lee JH, Perocchi F, Chen W, Clish CB, Ayata C, Brookes PS, Mootha VK. (2010) Nutrient-sensitized screening for drugs that shift energy metabolism from mitochondrial respiration to glycolysis. Nature Biotechnol. 28: 249-255. PMID 20160716
    • Wojtovich AP, Williams DM, Karcz MK, Lopes CM, Gray DA, Nehrke KW, Brookes PS. (2010) A novel mitochondrial K(ATP) channel assay. Circ Res. 106: 1190-1196. PMID 20185796
    • Caito S, Rajendrasozhan S, Cook S, Chung S, Yao H, Friedman AE, Brookes PS, Rahman I. (2010) SIRT1 is a redox-sensitive deacetylase that is post-translationally modified by oxidants and carbonyl stress. FASEB J. 24: 3145-3159. PMID 20385619
    • Chouchani ET, Hurd TR, Nadtochiy SM, Brookes PS, Fearnley IM, Lilley KS, Smith RA, Murphy MP. (2010) Identification of S-nitrosated mitochondrial proteins by S-nitrosothiol difference in gel electrophoresis (SNO-DIGE): implications for the regulation of mitochondrial function by reversible S-nitrosation. Biochem J. 430: 49-59. PMID 20533907
    • Gunter TE, Gerstner B, Lester T, Wojtovich AP, Malecki J, Swarts SG, Brookes PS, Gavin CE, Gunter KK. (2010) An analysis of the effects of Mn2+ on oxidative phosphorylation in liver, brain, and heart mitochondria using state 3 oxidation rate assays. Toxicol Appl Pharmacol. 249: 65-75. PMID 20800605
    • Wojtovich AP, Nehrke KW, Brookes PS. (2010) The mitochondrial complex II and ATP-sensitive potassium channel interaction: quantitation of the channel in heart mitochondria. Acta Biochim Pol. 57: 431-434. PMID 21103454


    • Skalska J, Brookes PS, Nadtochiy SM, Hilchey SP, Jordan CT, Guzman ML, Maggirwar SB, Briehl MM, Bernstein SH. (2009) Modulation of cell surface protein free thiols: a potential novel mechanism of action of the sesquiterpene lactone parthenolide. PLoS One. 4: e8115. PMID 19956548
    • Nadtochiy SM, Burwell LS, Ingraham CA, Spencer CM, Friedman AE, Pinkert CA, Brookes PS (2009) In vivo cardioprotection by S-nitroso-2-mercaptopropionyl glycine. J Mol Cell Cardiol. 46: 960-968. PMID 19339206
    • Ingraham CA, Burwell LS, Skalska J, Brookes PS, Howell RL, Sheu S-S, Pinkert CA. (2009) NDUFS4: Creation of a mouse model mimicking a complex I disorder. Mitochondrion. 9: 204-210. PMID 19460290
    • Prime TA, Blaikie FH, Nadtochiy SM, James AM, Dahm CC, Vitturi D, Patel RP, Hiley CR, Abakumova I, Requejo R, Chouchani E, Hurd TR, Garvey J, Taylor CT, Brookes PS, Smith RAJ, Murphy MP. (2009) A mitochondria-targeted S-nitrosothiol reversibly modulates respiration, selectively nitrosates mitochondrial thiol proteins and protects against cardiac ischemia-reperfusion injury. Proc. Natl. Acad. Sci. USA. 106: 10764-10769. PMID 19528654
    • Hoffman DL, Brookes PS. (2009) Oxygen sensitivity of mitochondrial reactive oxygen species generation depends on metabolic conditions. J. Biol. Chem. 284: 16236-16245. PMID 19366681
    • Burwell LS, Nadtochiy SM, Brookes PS. (2009) Cardioprotection by metabolic shut-down and gradual wake-up. J. Mol. Cell. Cardiol. 46: 804-810. PMID 19285082
    • Nadtochiy SM, Burwell LS, Ingraham CA, Pinkert CA, Brookes PS (2009) In vivo cardioprotection by S-nitroso-2-mercaptopropionyl glycine. J. Mol. Cell. Cardiol. 46: 960-968. PMID 19339206
    • Schopfer FJ, Batthyany C, Baker PRS, Bonacci G, Cole MP, Rudolph V, Groeger AL, Rudolph TK, Nadtochiy SM, Brookes PS, Freeman BA (2009) Detection and quantification of protein adduction by electrophilic fatty acids: mitochondrial generation of fatty acid nitroalkene derivatives. Free Radic. Biol. Med. 46: 1250-1259. PMID 19353781
    • Nadtochiy SM, Baker PR, Freeman BA, Brookes PS. (2009) Mitochondrial nitroalkene formation and mild uncoupling in ischemic preconditioning: implications for cardioprotection. Cardiovasc Res. 82: 333-340. PMID 19050010
    • Wojtovich AP, Brookes PS. (2009) The complex II inhibitor atpenin A5 protects against cardiac ischemia-reperfusion injury via activation of mitochondrial KATP channels. Basic Res. Cardiol. 104: 121-129. PMID 19242645


    • Nadtochiy SM, Nauduri D, Shimanskaya TV, Sagach VF, Brookes PS. (2008) Purine release: a protective signaling mechanism of the mitochondrial permeability transition pore in ischemia. Fiziol. Zh. 54: 5-14. PMID 19227234
    • Wojtovich AP, Burwell LS, Sherman TA, Nehrke KW, Brookes PS. (2008) The C. elegans mitochondrial KATP channel: A potential target for preconditioning. Biochem. Biophys. Res. Commun. 376: 625-628. PMID 18809388
    • Burwell LS, Brookes PS. (2008) Mitochondria as a target for the cardioprotective effects of nitric oxide in ischemia reperfusion injury. Antiox. Redox Signal. 10: 579-599. PMID 18052718
    • Brookes PS, Hoffman DL. (2008) Aging & Cardiac Ischemia: Mitochondrial Free Radical Considerations. Chapter 14 in “Oxidative Stress in Aging: From Model Systems to Human Diseases”. (Eds. S Miwa, K Beckman, F Muller). Springer Verlag, NY.
    • Brookes PS, Wojtovich AP, Burwell LS, Hoffman DL, Nadtochiy SM. (2008) The interaction of mitochondrial membranes with reactive oxygen and nitrogen species. In “Free Radical Effects on Membranes, Vol. 61 of Current Topics in Membranes”. (Eds. S Matalon, S Simon, D Benos). Elsevier, NY.
    • Brookes PS, Parker N, Buckingham JA, Vidal-Puig A, Halestrap AP, Gunter TE, Nicholls DG, Bernardi P, Lemasters JJ, Brand MD. (2008) UCPs: Unlikely Calcium Porters. Nature Cell Biol. 10: 1235-1237. PMID 18978830
    • Wojtovich AP, Brookes PS. (2008) The endogenous mitochondrial complex II inhibitor malonate regulates mitochondrial ATP-sensitive potassium channels: Implications for ischemic preconditioning. Biochim. Biophys. Acta. 1777: 882-889. PMID 18433712
    • Guijarro A, Osei-Hyiaman D, Harvey-White J, Kunos G, Suzuki S, Nadtochiy S, Brookes PS, Meguid MM. (2008) Sustained weight loss after Roux-en-Y gastric bypass is characterized by down regulation of endocannabinoids and mitochondrial function. Ann. Surg. 247: 779-790. PMID 18438115


    • Guijarro A, Suzuki S, Chen C, Kirchner H, Middleton FA, Nadtochiy SM, Brookes PS, Niijima A, Inui A Meguid MM. (2007) Characterization of weight loss and regain after Roux-en-Y gastric bypass in rats. Am. J. Physiol. 293: R1474-R1489. PMID 17626126
    • Brookes PS, Morse K, Ray DM, Tompkins AJ, Young SM, Hilchey S, Salim S, Konopleva M, Andreeff M, Phipps R, Bernstein SH. (2007) The triterpenoid CDDO and its derivatives elicit human lymphoid cell apoptosis through a novel pathway involving the unregulated mitochondrial permeability transition pore. Cancer Res. 67:1793-1802. PMID 17308122
    • Brookes PS. (2007) Mitochondrial production of oxidants and their role in the regulation of cellular processes. Chapter 5.3 in “Handbook of Neurochemistry and Molecular Neurobiology” 3rd Edition. Vol. 5. “Brain Energy Metabolism.” (Eds. G. Gibson & G. Dienel), Kluwer, NY.
    • Shiva S, Brookes PS, Darley-Usmar VM. (2007) Methods for measuring the regulation of respiration by nitric oxide. In “Mitochondria”, Methods in Cell Biology Series (Eds. L Pon and Eric Schon), 80: 395-416. Acad. Press/Elsevier, San Diego CA. PMID 17445706
    • Nadtochiy SM, Burwell LS, Young SM, Brookes PS. (2007) Cardioprotection and mitochondrial S-nitrosation: effects of S-nitroso-2-mercaptopropionyl glycine (SNO-MPG) in cardiac ischemia-reperfusion injury. J. Mol. Cell. Cardiol. 42:812-825. PMID 17350035
    • Shiva S, Sack MN, Greer JJ, Duranski M, Ringwood LA, Burwell LS, Wang X, MacArthur PH, Shoja A, Raghavachari N, Calvert JW, Brookes PS, Lefer DJ, Gladwin MT. (2007) Nitrite augments tolerance to ischemia-reperfusion injury via the modulation of mitochondrial electron transfer. J. Exp. Med. 204:2089-2102. PMID 17682069
    • Hoffman D, Salter JD, Brookes PS. (2007) The response of mitochondrial reactive oxygen species generation to steady-state oxygen tension: implications for hypoxic cell signaling. Am. J. Physiol. 292:H101-108. PMID 16963616


    • Nadtochiy SM, Tompkins AJ, Brookes PS. (2006) Different mechanisms of mitochondrial proton leak in ischemia-reperfusion injury and preconditioning: implications for cardioprotection and pathology. Biochem. J. 395:611-618. PMID 16436046
    • Burwell LS, Nadtochiy SM, Tompkins AJ, Young S, Brookes PS. (2006) Direct evidence for S-nitrosation of mitochondrial complex I. Biochem. J. 394:627-634. PMID 16371007
    • Brookes PS, Freeman RA, Barone MC. (2006) A short-cut to mitochondrial signaling and pathology. Free Rad. Biol. Med. 41:41-45. PMID 16781451


    • Itoh S, Lemay S, Osawa M, Che W, Berdirian A, Tompkins A, Brookes PS, Yan C, Abe J-I. (2005) Mitochondrial Dok-4 recruits Src kinase and regulates mitochondria-derived reactive oxygen species (ROS) and subsequent NF-kB activation in endothelial cells. J. Biol. Chem. 280:26383-26396. PMID 15855164
    • Brand MD, Pakay JL, Ocloo A, Kokoszka J, Wallace DC, Brookes PS, Cornwall EJ. (2005) The basal proton conductance of mitochondria depends on adenine nucleotide translocase content. Biochem J. 392:353-362. PMID 16076285
    • Tompkins AJ, Burwell LS, Digerness SB, Holman WL, Brookes PS. (2005) Mitochondrial dysfunction in cardiac ischemia-reperfusion injury: ROS from complex I, without inhibition. Biochim. Biophys. Acta. 1762:223-231. PMID 16278076


    • Shiva S, Crawford JH, Ramachandran A, Ceaser EK, Hillson T, Brookes PS, Patel R, Darley-Usmar VM. (2004) Mechanisms of the interaction of nitroxyl with mitochondria. Biochem. J. 379:357-366. PMID 14723605
    • Brookes PS, Yoon Y, Robotham JL, Anders MW, Sheu SS. (2004) Ca2+, ATP & ROS – a mitochondrial love/hate triangle. Am. J. Physiol. 287:C817-833. PMID 15355853
    • Brookes PS. (2004) Mitochondrial H+ Leak and ROS generation: an odd couple. Free Rad. Biol. Med. 38:12-23. PMID 15589367
    • Shiva S, Moellering D, Ramachandran A, Levonen AL, Landar A, Venkatraman A, Ceaser E, Ulasova E, Crawford JH, Brookes PS, Patel RP, Darley-Usmar VM. (2004) Redox signaling: from nitric oxide to oxidized lipids. Biochem. Soc. Symp. 71:107-120. PMID 15777016
    • Brookes PS, Darley-Usmar VM. (2004) Role of calcium and superoxide dismutase in sensitizing mitochondria to peroxynitrite-induced permeability transition. Am. J. Physiol. 286:H39-H46. PMID 12933349
    • Brookes PS. (2004) Mitochondrial nitric oxide synthase. Mitochondrion 3:187-204. PMID 16120354


    • Davies JE, Digerness SB, Goldberg SP, Killingsworth CR, Katholi CR, Brookes PS, Holman WL. (2003) Intra-myocyte ion homeostasis during ischemia-reperfusion injury: effects of pharmacologic preconditioning and controlled reperfusion. Ann. Thorac. Surg. 76:1252-1258. PMID 14530020
    • Digerness SB, Brookes PS, Goldberg SP, Katholi CR, Holman WL. (2003) Modulation of mitochondrial adenosine triphosphate-sensitive potassium channels and sodium-hydrogen exchange provide additive protection from severe ischemia-reperfusion injury. J. Thorac. Cardiovasc. Surg. 125:863-871. PMID 12698150
    • Brookes PS, Kraus DW, Shiva S, Doeller JE, Barone MC, Patel RP, Lancaster JR, Darley-Usmar VM. (2003) Control of mitochondrial respiration by NO”, effects of low oxygen & respiratory state. J. Biol. Chem. 278:31603-31609. PMID 12788936
    • Venkatraman A, Shiva S, Davis AJ, Bailey SM, Brookes PS, Darley-Usmar VM. (2003) Chronic alcohol consumption increases the sensitivity of rat liver mitochondrial respiration to inhibition by nitric oxide. Hepatology 38:141-147. PMID 12829996
    • Barone MC, Darley-Usmar VM, Brookes PS. (2003) Reversible inhibition of cytochrome c oxidase by peroxynitrite proceeds through ascorbate-dependent generation of nitric oxide. J. Biol. Chem. 278:27520-27524. PMID 12743113


    • Brookes PS, Pinner AL, Ramachandran A, Coward L, Barnes S, Kim H, Darley-Usmar VM. (2002) High-throughput 2D blue-native electrophoresis, a tool for functional proteomics of mitochondria and cell-signaling complexes. Proteomics. 2:969-977. PMID 12203892
    • Brookes PS, Digerness SB, Parks DA, Darley-Usmar VM. (2002) Mitochondrial function in response to cardiac ischemia-reperfusion after oral treatment with quercetin. Free Rad. Biol. Med. 32:1220-1228. PMID 12031906
    • Lin T-K, Hughes G, Muratovska A, Blaikie FH, Brookes PS, Darley-Usmar VM, Smith RAJ, Murphy MP. (2002) Specific modification of mitochondrial protein thiols in response to oxidative stress: a proteomics approach. J. Biol. Chem. 277:17048-17056. PMID 11861642
    • Brookes PS, Baggot JE. (2002) Oxidation of 10-formyl-tetrahydrofolate by complex IV of rat mitochondria. Biochemistry. 41:5633-5636. PMID 11969424
    • Brookes PS, Shiva S, Patel RP, Darley-Usmar VM. (2002) Measurement of mitochondrial respiratory thresholds and the control of respiration by nitric oxide. Meth. Enzymol. 359:305-319. PMID 12481582
    • Brookes PS, Levonen A-L, Shiva S, Sarti P, Darley-Usmar VM. (2002) Mitochondria: Regulators of signal transduction by reactive oxygen and nitrogen species. Free Rad. Biol. Med. 33:755-764. PMID 12208364
    • Cooper CE, Patel RP, Brookes PS, Darley-Usmar VM. (2002) Nano-transducers in redox cell signaling; modification of thiols by reactive oxygen and nitrogen species. Trends Biochem. Sci. 27:489-492. PMID 12368076
    • Ramachandran A, Levonen A-L, Brookes PS, Ceaser E, Shiva S, Barone M-C, Darley-Usmar VM. (2002) Mitochondria, Nitric Oxide, and Cardiovascular Dysfunction. Free Rad. Biol. Med. 33:1465-1474. PMID 12446203
    • Brookes P, Darley-Usmar VM. (2002) Hypothesis: The mitochondrial NO signaling pathway, and the transduction of nitrosative to oxidative cell signals: an alternative function for cytochrome c oxidase. Free Rad. Biol. Med. 32:370-374. PMID 11841927


    • Dai L, Brookes PS, Darley-Usmar VM, Anderson PG. (2001) Bioenergetics in cardiac hypertrophy: mitochondrial respiration as a pathological target of nitric oxide. Am. J. Physiol. 281:H2261-2269. PMID 11709391
    • Shiva S, Brookes PS, Patel RP, Anderson PG, Darley-Usmar VM. (2001) Nitric oxide partitioning into mitochondrial membranes and the control of respiration at cytochrome c oxidase. Proc. Natl. Acad. Sci. USA 98:7212-7217. PMID 11416204
    • Brookes PS, Zhang J, Dai L, Zhou F, Parks DA, Darley-Usmar VM, Anderson PG. (2001) Increased sensitivity of mitochondrial respiration to inhibition by nitric oxide in cardiac hypertrophy. J. Mol. Cell. Cardiol. 33:69-82. PMID 11133224
    • Levonen A-L, Patel RP, Brookes P, Go Y-M, Jo H, Parthasarathy S, Darley-Usmar VM. (2001) Mechanisms of cell signaling by nitric oxide and peroxynitrite: implications for the modulation of cellular oxidative and nitrosative stress. Antiox. Redox Signaling. 3:215-229. PMID 11396477


    • Brookes PS, Padilla-Salinas E, Darley-Usmar K, Eiserich JP, Freeman BA, Darley-Usmar VM, Anderson PG. (2000) Concentration dependent effects of nitric oxide on mitochondrial permeability transition and cytochrome c release. J. Biol. Chem. 275:20474-20479. PMID 10791954
    • Brookes PS, Patel RP, Levonen A-L, Shiva S, Moellering D, Anderson PG, Jo H, Darley-Usmar VM. (2000) Cytoprotective mechanisms of nitric oxide: the role of cell signaling. In “Free Radicals in Chemistry, Biology and Medicine”. (Eds. T. Yoshikawa, S. Toyokuni, Y. Yamamoto and Y. Niato.) OICA International, London, UK. ISBN 1903063043


    • Brookes PS, Bolanos JP, Heales SJR. (1999) The assumption that nitric oxide inhibits mitochondrial ATP synthesis is correct. FEBS Lett. 446:261-263. PMID 10100854


    • Changani, KK, Bell JD, Brooks KJ, Fuller BJ, Taylor-Robinson SD, Davidson BR, Brookes PS, Bates TE. (1998) Depression of liver mitochondrial complex II-linked respiration following cooling: beneficial effects of using a prostacyclin analogue during hypothermia. Cryo-Letters 19:71-78
    • Brookes PS, Land JM, Clark JB, Heales SJR. (1998) Peroxynitrite and brain mitochondria: evidence for increased proton leak. J. Neurochem. 70:2195-2202. PMID 9572308
    • Brookes PS, Buckingham JA, Tenreiro AM, Hulbert AJ, Brand MD. (1998) The proton permeability of the inner membrane of liver mitochondria from exothermic and endothermic vertebrates and from obese rats: correlations with standard metabolic rate and phospholipid fatty acid composition. Comp. Biochem. Physiol. 119B:325-334. PMID 9629666
    • Heales SJR, Bolaños JP, Stewart VC, Brookes PS, Land JM, Clark JB. (1998) Nitric oxide, Mitochondria & Neurological disease. Biochim. Biophys. Acta 1410:215-228. PMID 10076028


    • Brookes PS, Land JM, Clark JB, Heales SJR. (1997) Stimulation of glyceraldehyde-3-phosphate dehydrogenase by oxyhemoglobin. FEBS Lett. 416:90-92. PMID 9369240
    • Brookes PS, Hulbert AJ, Brand MD. (1997) The proton permeability of liposomes made from mitochondrial inner membrane phospholipids: no effect of fatty acid composition. Biochim. Biophys. Acta 1330:157-164. PMID 9408168
    • Brookes PS, Rolfe DFS, Brand MD. (1997) The proton permeability of liposomes made from mitochondrial inner membrane phospholipids: comparison with intact mitochondria. J. Memb. Biol. 155:167-174. PMID 9049110


    • Brookes PS, Rolfe DFS, Brand MD. (1996) Proton leak of mitochondrial inner membrane phospholipid liposomes. In Biothermokinetics of the living cell. (Eds. HV Westerhoff et al.) pp3-10. BTK Press, Amsterdam, NL.